The quality of cell growth is good, why haven't the expression levels of viruses and proteins increased?
If the bacterial and viral strains pass all the tests and can be used for vaccine production, the quality of cell growth is undoubtedly a necessary condition for high expression.
Vaccines produced using cells involve culturing a certain amount of animal cells in vitro and inoculating them with a virus. The virus is then used to proliferate within the cells to obtain the desired viral antigen, which is then used to produce the vaccine. The quality of cell growth includes cell density and morphology, and without good cell growth quality, it is impossible to have high expression levels.
However, it should be noted that when the cell growth quality is good and the density and thickness are large, the viral inoculation amount should also increase accordingly, and the expression level will only increase. Otherwise, the high-quality cell culture in the early stage may be wasted.
At the same time, the expression and secretion of viruses and proteins may become saturated at a certain concentration, so in order to achieve high expression, it is necessary to study the appropriate collection time and frequency.
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