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How to adjust the pH value with sodium bicarbonate?

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As shown in the figure, the pH value of the culture medium increases parabolically with the increase of sodium bicarbonate content. When a certain amount of sodium bicarbonate is added, the pH change becomes smaller and smaller. The osmotic pressure value of the culture medium increases linearly with the increase of sodium bicarbonate content, and the larger the amount of sodium bicarbonate, the higher the osmotic pressure value.

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Is pollution caused by poor quality of the culture medium?

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The culture medium is not a sterile product, but there is control over the number of bacterial colonies.

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Is L-glutamine important in cell culture? Is it unstable in solution?

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L-glutamine is important in cell culture. After removing the amino group, L-glutamine can serve as an energy source for cultured cells and participate in protein synthesis and nucleic acid metabolism.

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The quality of cell growth is good, why haven't the expression levels of viruses and proteins increased?

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If the bacterial and viral strains pass all the tests and can be used for vaccine production, the quality of cell growth is undoubtedly a necessary condition for high expression.